FIELD STUDY ADVANCED MOLECULAR BIOLOGY LAB

Location:  2185 E Mall, UBC  Vancouver, BC V6T 1Z4
Time:  8:45Am for attendance
THIS IS A MANDATORY ALL DAY FIELD TRIP ENDING AT 230 OR 3PM To find the Michael Smith Lab, First go to UBC BOOKSTORE and it is DIRECTLY SOUTH beside the bookstore.

THEIR WEBSITE is here

Summary Notes and Worksheets - Quiz next day!

NOTES: a summary of what we learned about protein synthesis
A diagram worksheet summary
and a key to the Protein synthesis worksheet we did last day

More practice generating mRNA, tRNA and Amino Acids

Assignment
Draw a cartoon summarizingTranscription and Translation

Here's two other explanations of transcription and translation

We did some lessons on Protein synthesis- Review these steps

TRANSCRIPTION

1.      Using the DNA as a template, RNA polymerase synthesizes the messenger

RNA from the 5’to 3’ direction 

2.     The messenger RNA is protected with a 5’ cap and a polyA tail

3.     The introns (or junk messages) are edited out of the strand

4.     The exons (or essential messages) are kept in

Now the mRNA is ready to move out to the cytoplasm to be translate By the ribosome.

TRANSLATION

PROTEIN SYNTHESIS TAKES PLACE

AT THE RIBOSOME, composed of

The LARGE RIBOSOMAL SUBUNIT AND

SMALL RIBOSOMAL SUBUNIT.

THE AMINO ACIDS ARE FETCHED BY

THE tRNA (transfer RNA) and tRNA has the ANTIcodon,

 the complement for the mRNA.

The tRNA goes to a binding site on the ribosome and

they release their amino acid.  The ribosome synthesizes

the polypeptide by using dehydration synthesis.

The start codon is always, universally  AUG.  The start anticodon is

Always UAC.  And the start amino acid is MET or methionine.

When the start codon gets a tRNA, it is called INITIATION.

WHEN THE POLYPEPTIDE CHAIN IS GENERATED ,it is called

ELONGATION

When the RELEASE FACTOR LANDS ON THE STOP CODON,

 it is called TERMINATION.

DIGESTION, your test is in one week on January 9

Slideshow1
Slideshow2

Your test will be based on your study notes
Studynotes answerkey1
Studynotes answerkey2

Enzymes and Hormones
Enzymes and Hormones Answers

attribution


Labs 
Lab 1 Transit Time lab and Salivary Amylase demonstration
Lab 2  Observing morphology of an animal stomach sample

Writing up your lab report.
You may be in a group of two or three.  But you must state who wrote which part of write up.
Lab Write up Format

Intro to Digestion

Now that you have a better idea of how enzymes work, we will start on the human body system.  These are theDigestion Notes.
We will go over this in class

Assignment:  Using 11 by 17 paper (from photocopy room) draw a map of the digestive system and label what happens in each of the organs

Enzyme Worksheets and please write up our labs on Liver Latte and Dry Ice

Work on these enzyme worksheets please
Worksheet 1
Worksheet 2

and Write up labs for the dry ice experiment and the Liver Latte lab
The write ups ought to include

Names (of no more than two authors)
Date
Title
Headings are:  Purpose, Materials, Procedure, Observations, Conclusion. For the Dry Ice Lab. Describe all your observations of the properties of carbon dioxide.

 include the Equilibrium equation showing what happens when carbon dioxide is mixed with water.  For the Liver Latte Lab, include the chemical equation for the decomposition of hydrogen pyroxide.  Use key words from our enzyme unit/

November 22 Biology Notes

Review these notes on enzymes

ENZYMES BIOLOGY 12

See this presentation 
And do this reading

Metabolism is the sum total of biochemical reactions in an organism it involves building things, anabolism , and breaking stuff down, catabolism.

Students will prepare their own summary consisting of two pages which summarize all key words in a cartoon of whatever is covered today

Next have students work through these worksheets
Enzyme worksheet 1
Enzyme practice questions